The Rorrer Group laboratory is set up
to conduct bioprocess engineering research. The laboratory is fully
equipped for cell culture development
and bench-scale bioreactor culture. The laboratory also has a variety
of analytical equipment to support bioprocess engineering research
activities, including GC and HPLC.
Laboratory Space
Approximately 700 ft2 of climate-controlled laboratory space in Gleeson
Hall (Rooms 302 and 309) at Oregon State University. The laboratory space
also features a separate dishroom and chemicals storage room, two 4 ft
fume hoods, a steam autoclave, and a deionized water source.
Major Equipment
[link to photos of selected equipment]
Bioreactors
- Two-liter airlift photobioreactors (4 total)
- Jacketed Bellco bioreactors (7 total; 0.1 -1.0 liters)
- Two-liter cell culture fermenter, Applikon
- Two-liter fermenter, VirTis Omni-Culture
- Three-liter tubular recycle photobioreactor
- Multi-channel peristaltic pumps (3 total; Masterflex, Buchler)
- Circulating/chilling water baths (4 total; Lauda, Neslab)
Cell and Tissue Culture
- Full-size, low-temperature illuminated incubators (5 total; VWR,
Precision Scientific)
- Temperature-controlled environmental orbital shakers, Lab-line 3520
(2)
- Benchtop orbital shakers (2 total, Labline, VWR)
- Laminar-flow sterile hood, 3 ft, Baker EG3252
- Photosynthetic OER apparatus (YSI) and computer with Labtech Notebook
Analytical Equipment
- Dionex DX 300 gradient HPLC, with UV-VIS & conductivity detectors
- Waters isocratic HPLC, with Waters 501 pump, Waters 484 UV-VIS detector,
Altex 154 RI detector
- Gilson fraction collector for HPLC
- SRI Chromatography computer
- Hewlett-Packard HP 5791 Series II gas chromatograph with FID & TCD
detectors; HP Vectra 486 PC with Chemstation software
- UV-VIS spectrophotometer, Hitachi
- ELISA plate reader, BioTek EL311SX
- ELISA manual plate washer system, BioTek
- BET surface area and micropore analysis system, Micromeretics ASAP-2000
- Coulter
Counter
- pH/ISE/conductivity meters (5 total, Corning, Orion, Markson)
- D.O. meters (3 total, Virtis, YSI)
Microscopy
- Light microscope, 100-430X, AO/Spenser
- Stereo dissecting microscope, 7-42X, American Optical
Reactors
500 mL Parr 4561 stirred autoclave pressure reactor with temperature
controller and tachometer
Laboratory Support
- Centrifuge, IEC Centra 4B
- Microfuge, Eppendorf
- Analytical balance, Mettler AJ100
- Top loading balances, Sartorius (2)
- Refrigerator / -20 oC freezers (3)
- Laboratory oven, VWR Scientific
- Autoclave, 16 inch, American Sterilizer
- Reacti-Therm sample prep. Module, Pierce
- Sonicating bath, Branson
- Quantum flux meter, LI-COR
- Digital tachometer, Cole-Parmer
- 10-channel digital thermometers, Omega (2)
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