| We
have developed axenic cell and tissue cultures
for representative species of red, brown, and green marine macroalgae. For red macroalgae
of terete thallus moropholgy, callus induction and shoot tissue regeneration
techniques are used to establish asexual, vegetatively
propagated cell and tissue cultures. For
brown macroalgae in the kelp family, cell cultures
are established by isolation and vegetative propagation of the microscopic
female gametophyte life phase. All culture systems are photosynthetic, requiring
light and CO2 for growth. The
tissues are freely suspended in a seawater based nutrient medium, but individual
tissues or cell clumps are not friable, and so the biomass must be mechanically
dissociated prior to subculture. |
