Higgins, AZ, Karlsson, JOM, Curve fitting approach for measurement of cellular osmotic properties by the electrical sensing zone method. I. Osmotically inactive volume. Cryobiology. 57: 223-233, 2008.
Higgins, AZ, Karlsson, JOM, Coincidence Error During Measurement of Cellular Osmotic Properties by the Electrical Sensing Zone Method. Cryo-Letters. 29(6): 447-461, 2008.
Higgins, AZ, Karlsson, JOM, Curve fitting approach for measurement of cellular osmotic properties by the electrical sensing zone method. II. Membrane Water Permeability. Cryobiology. In press.
Higgins, AZ, Karlsson, JOM, Measurement of Cell Membrane Permeability in Adherent Cultures of Pancreatic Beta-Cells by Fluorescence Quenching. Journal of Membrane Biology. In preparation.
Higgins, AZ, Karlsson, JOM, Effect of Intercellular Junction Protein Expression on Intracellular Ice Formation. Biophysical Journal. In preparation.
Bower, CK, Parker, JE, Higgins, AZ, Oest, ME, Wilson, JT, Valentine, BA, Bothwell, MK, McGuire, J. Protein antimicrobial barriers to bacterial adhesion: in vitro and in vivo evaluation of nisin-treated implantable materials. Colloids and Surfaces B: Biointerfaces. 25: 81-90, 2002.
Refereed Conference Proceedings/Abstracts
Fry, AK, Higgins, AZ. Determination of cryoprotectant permeability properties in monolayers of bovine endothelial cells using an in situ fluorescence quenching technique. Cryobiology. In press.
Higgins, AZ, Karlsson, JOM. Effect of Hold Temperature and Cooling Rate on Intracellular Ice Formation in Micropatterned Tissue Constructs. Cryobiology. 57(3): 326, 2008.
Higgins, AZ, Karlsson, JOM. Effect of Intercellular Junction Protein Expression on Intracellular Ice Formation in Genetically Modified Pancreatic Beta-Cells. Cryobiology. 55(3): 330, 2007.
Higgins, AZ, Karlsson, JOM. Comparison of Membrane Permeability Properties in Monolayers and Suspensions of Bovine Endothelial Cells. Cryobiology. 55(3): 375, 2007.
Higgins, AZ, Stott, SL, Karlsson, JOM. Effect of Instrument Dynamic Range on the Estimation of Osmotic Properties Using Electronic Cell Sizing Techniques. Cryobiology. 53(3): 444-445, 2006.
Higgins, AZ, Karlsson, JOM. Quantification of Tissue Dehydration by Measurement of Fluorescence Quenching. Cryobiology. 51(3): 413, 2005.
Conference Oral Presentations
Fry, AK, Higgins, AZ. Determination of cryoprotectant permeability properties in monolayers of bovine endothelial cells using an in situ fluorescence quenching technique. Cryobiology Conference, Sapporo, Japan. July 19-23, 2009.
Higgins, AZ, Karlsson, JOM. Effect of Hold Temperature and Cooling Rate on Intracellular Ice Formation in Micropatterned Tissue Constructs. Cryobiology Conference, Charlotte, NC. July 20-23, 2008.
Higgins, AZ, Karlsson, JOM. Effect of Intercellular Junction Protein Expression on Intracellular Ice Formation in Genetically Modified Pancreatic Beta-Cells. Cryobiology Conference, Lake Louise, Canada. July 29-Aug 1, 2007.
Higgins, AZ, Karlsson, JOM. Kinetics of Membrane Water Transport in Pancreatic Tissue. Regenerate International Conference and Exposition. Atlanta, GA. June 2-3, 2005.
Conference Poster Presentations
Fry, A, Elder, R, Lowery, N, Higgins, AZ. In Situ Measurement of Cryoprotectant Permeability in Cultured Endothelial Cells and Applications to Cryopreservation. Annual Meeting of the Biomedical Engineering Society, Pittsburgh, PA. October 7-10, 2009.
Higgins, AZ, Karlsson, JOM. Comparison of Membrane Permeability Properties in Monolayers and Suspensions of Bovine Endothelial Cells. Cryobiology Conference, Lake Louise, Canada. July 29-Aug 1, 2007.
Higgins, AZ, Stott, SL, Karlsson, JOM. Effect of Instrument Dynamic Range on the Estimation of Osmotic Properties Using Electronic Cell Sizing Techniques. Cryobiology Conference, Hamburg, Germany. July 24-27, 2006.
Higgins, AZ, Karlsson, JOM. Quantification of Tissue Dehydration by Measurement of Fluorescence Quenching. Cryobiology Conference, Minneapolis, MN. July 24-27, 2005.
Higgins, AZ, Karlsson, JOM. Cell Membrane Water Permeability and Activation Energy in Hepatic Tissue. Hilton Head Engineering Tissues Workshop, Hilton Head, SC. March 9-13, 2005.
Higgins, AZ, Karlsson, JOM. Factors Affecting the Viability of Neural Tissue After Cryopreservation. Tissue Engineering Society International Annual Meeting, Lausanne, Switzerland. October 10-13, 2004.
Higgins, AZ, Karlsson, JOM. Fluorescence Self-Quenching Technique to Quantify Tissue Dehydration During Cryopreservation. Tissue Engineering Society International Annual Meeting, Lausanne, Switzerland. October 10-13, 2004.
Higgins, AZ, Karlsson, JOM. Fluorescence Quenching Technique for Measurement of Mass Transport During Tissue Preservation. Georgia Tech Emory Center for the Engineering of Living Tissues Educational Partners Symposium. Atlanta, GA. October 5-6, 2004.
Higgins, AZ, Karlsson, JOM. Measurement of Water Transport in Cell Monolayers Using Calcein Self-Quenching. Georgia Tech Emory Center for the Engineering of Living Tissues NSF Site Visit Meeting. Atlanta, GA. April 13-15 2004.
Higgins, AZ, Karlsson, JOM. Cryopreservation of Primary Cortical Neurons. Georgia Tech Emory Center for the Engineering of Living Tissues Educational Partners Symposium. Atlanta, GA. October 19-21, 2003.
Bower, CK, Parker, JE, Higgins, AZ, Oest, ME, Wilson, JT, Valentine, BA, Bothwell, MK, McGuire, J. Protein antimicrobial barriers to bacterial adhesion: in vitro and in vivo evaluation of nisin-treated implantable materials. American Chemical Society National Meeting. Orlando, FL. April 7-11, 2002.
Invited Seminars (non-conference)
Long-term stabilization of living cells and tissues by cryopreservation. Oregon State University, Materials Science Program Seminar Series, Corvallis, OR, April 30, 2009.
Factors affecting intracellular ice formation during tissue cryopreservation. Oregon Health and Science University, Biomedical Engineering Seminar Series, Portland, OR, Jan. 9, 2009.
Measurement of cellular osmotic properties by the Coulter counter method and applications to cryopreservation. The Coulter Principle: Foundations and Advanced Applications. Harvard University, Boston, MA, Oct. 15, 2008.
High-speed imaging of biological samples. Bioengineering and Biosciences Unified Graduate Student Technique Symposium, Atlanta, GA, June 26-28, 2006.
Adam Higgins School of Chemical, Biological and Environmental Engineering Oregon State University Corvallis, OR 97331
